Amylase activity is determined using a coupled enzymatic assay, which results in a colorimetric 405 nm product, proportional to the amount of substrate, ethylidenepnpg7. Alpha amylase inhibitor an overview sciencedirect topics. Alphaamylase inhibition potential of the morinda lucida extracts was determined figure 1. This assay protocol is suitable for the colorimetric detection of amylase activity in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the amylase activity assay kit mak009. However, the hexane extract had only showed significant activity with 24. In our study, the hexane extract at a concentration 100. Antidiabetic activity of an ayurvedic formulation chaturmukha rasa. Plant phenolic and flavonoids compounds such as quercetin, ferulic acid, anthocyanins, catechin, and resveratrol were indicated in epidemiological studies to regulate glycemia via increased glucose uptake, insulin secretion, and inhibition of lipid peroxidation, alphaglucosidase, and alphaamylase 21, 22. Alpha amylase and alpha glucosidase inhibitory effects of. In vitro assay of alpha amylase inhibitory activity of some indigenous. Alpha amylase inhibitory activitv of some plant extracts with. Mtt assay was carried out to determine the potential antiproliferative and. The enzyme is very stable and can be incubated at 60c during the mashing process in the brewery. Invitro alpha amylase inhibitory activity of the leaf.
Briefly, 250 l of the 5 mgml extract was preincubated with 250 l of. Enzymatic, fluorometric assay of aamylase in serum. The inhibition of amylase by all the extracts at lower concentrations 0. This may be the mechanism by which chaturmukha rasa inhibits. Since it is unable to cleave branched linkages in branched polysaccharides such as. The starch solution 1% wv was obtained by boiling and stirring 1 g of potato starch in 100 ml of sodium phosphate buffer for 30 min. Enzymatic method for determining amylase activity amylase. At the same time, both ethanol and hexane extracts showed appreciable. Evaluation of alphaamylase inhibition by urtica dioica and juglans.
Request pdf in vitro assay of alpha amylase inhibitory activity of some indigenous plants diabetes mellitus is a metabolic disorder characterized by chronic. The ic 50 values of stem extract was found to be 73. The spectrophotometric stop reaction determination a 540, light path 1 cm is based on the following reaction unit definition. Understanding the mechanism of action and inhibition mechanism of. The percentage inhibition of assays showed maximum inhibitory effects 59. Amylase inhibitory activity of some traditionally used. It is also the major enzyme produced during the malting process. Amylase inhibitory assay, in vitro salivary amylase.